Authors: Clairton De Souza, Philip Polkinghorne, Auckland, New-Zealand


To maintain the intraocular pressure and simultaneous air exchange during the vitreous biopsy, preventing from dilution and contamination of the obtained sample. The method also facilitates collection of larger volumes of vitreous and is compatible with 25, 23 and 20-gauge systems.


The vitreous sample is collected in a gas-sterilized test tube by means of two 20-gauge Venflon intravenous cannulae. A male connector and a male/female connector placed in the aspiration tubing of the vitrectomy cutter complete the required circuit.

Effectiveness / Safety:

No patient developed complications such as intra and post-operative hypotony or retinal breaks in our experience of more than 50 patients in the last 6 years.