Difficulties of Surgical Procedure During Experimental Transplantation of Retinal Pigmented Epithelial Cells in a Large Animal Model



To report surgical difficulties during experimental transplantation of retinal pigmented epithelial cells in subretinal space on eyes of large animal model using nanofibers scaffold and injector.


Six eyes of minipigs underwent standard 3-port 23 G PPV. Implantation of nanofibers scaffold was performed through posterior retinomomy (2.5 mm) using new designed injector and additional 3.5 mm sclerotomy. At the end of the surgery endolaserphotocoagulation and SÖ-endotamponade were done. After the surgery thorough analysis of intraoperative complications and risk factors was performed in order to optimize the flow of surgery.


In all cases it was difficult to place the head of the minipig so that the iris would be in horizontal position. Excessive bleeding from episcleral vessels was often seen, but could be eliminated using cautery. Thick lens limited the vitrectomy procedure in all cases, that is why the vitreous was removed not completely. On the stage of creation of retinotomy the bleeding was minimized using endocautery but recurrent bleeding was seen after implantation of nanofibers scaffold. Additional 3.5 mm sclerotomy didn’t seem to cause any complications, such as hypotony, detachment of ciliary body or bleeding.


The implantation of nanofibers scaffold into porcine eye in vivo showed that the carrier can be implanted without significant damage of intraocular tissue and that the carrier maintains flat shape after placement into the subretinal space. Optimization of surgical technique of experimental transplantation of retinal pigmented epithelial cells in subretinal space with possible lens removal is a crucial in order to reach successful results, that could be reproduced on human eye.



Lyubomyr Lytvynchuk
Giessen, Germany
Email : lubko_l@yahoo.co.uk
Cell Phone: +4915174120180
Work Phone: +4915174120180