Andriy Sergienko, Luybomyr Lytvynchuk, Galina Lavrinchuk, Olena Vlasko (Kyiv, Ukraine)

Objective:

To evaluate the effects of vitreous and subretinal fluid on fibroblast-like cell strains, as a model of proliferative processes in vitro. To find out the evidences that PVR development is dependent not only on RPE migration.

Methods:

Eleven vitreous aspirates and 9 subretinal fluid samples, taken during PPV in eyes with retinal detachment, were added to cell strain L929 to evaluate the tendancy to proliferate permanently. Cellular effects were evaluated according to the changes in cell vital activity indices: survival, adhesion, mitotic index and polycaryocyte index. Vitreous aspirates induced a decrease in cellular adhesion and increase of mitotic activity. Subretinal fluid samples demonstrated a reduced cellular reproducibility of the cell strain.

Effectiveness / Safety:

Data analysis revealed the ability of subretinal fluid content to depress cellular reproducibility. Vitreous, on the other hand, revealed the ability to stimulate cellular mitosis, adhesion, and proliferation. The results suggest that there exists a favorable environment in vitreous cavity for PVR development.