Olena Vlasko, Andriy Sergienko, Lyubomyr Lytvynchuk, Galina Y. Lavrenchuk (Kyiv, Ukraine)

Advantages:

To study cellular effects of pharmacological agents (drugs, dyes and substitutes) on the highly proliferative fibroblast-like cell strain L929, as an in vitro model of proliferative vitreoretinopathy processes.

Methods:

Pharmacological agents for intravitreal administration (betamethasone, triamcynolone acetonide, mytomicine C, 5-fluoruracil, bevacizumab), dyes (ICG, brilliant blue, trypan blue) and vitreous substitutes (PFCL, silicon oil, viscoelastics) were added to tissue cultures of cell strain L929 in varying concentrations. The cellular effects were evaluated according to the changes noted in cellular vital activity indices: survival, mitotic index and polycaryocyte index and were compared to control samples. Every intravitreal drug revealed antiproliferative properties through the two main mechanisms: cytotoxicity and apoptosis. All dyes revealed biodegradable properties, but the blue dyes depressed slightly cellular mitosis. Silicon oil (1000 and 5000 cS) and methylcelulose expressed slight inhibition of cellular mitosis.

Effectiveness / Safety:

The cellular effects of pharmacological agents utilized during vitrectomy surgery, as revealed on fibroblast-like cells in tissue culture, allow a better understanding of the observed biological activity on vital tissues following surgery.